Unknown

Dataset Information

0

Pancreatic α and β cells are globally phase-locked.


ABSTRACT: The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic α and β cells play a crucial role in glucose homeostasis. However, how α and β cells coordinate to produce various Ca2+ oscillation patterns is still elusive. Using a microfluidic device and transgenic mice, we recorded Ca2+ signals from islet α and β cells, and observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. After a brief period of glucose stimulation, α and β cells' oscillations were globally phase-locked. While the activation of α cells displayed a fixed time delay of ~20 s to that of β cells, β cells activated with a tunable period. Moreover, islet α cell number correlated with oscillation frequency. We built a mathematical model of islet Ca2+ oscillation incorporating paracrine interactions, which quantitatively agreed with the experimental data. Our study highlights the importance of cell-cell interaction in generating stable but tunable islet oscillation patterns.

SUBMITTER: Ren H 

PROVIDER: S-EPMC9240067 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2602598 | biostudies-literature
| S-EPMC8308288 | biostudies-literature
| S-EPMC9882905 | biostudies-literature
| S-EPMC3558471 | biostudies-literature
| S-EPMC4910116 | biostudies-literature
| S-EPMC6048053 | biostudies-literature
| S-EPMC2877635 | biostudies-other
| S-EPMC5358097 | biostudies-literature
| S-EPMC7523508 | biostudies-literature
2013-01-30 | E-GEOD-38360 | biostudies-arrayexpress