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Diverse responses of pqqC- and phoD-harbouring bacterial communities to variation in soil properties of Moso bamboo forests.

ABSTRACT: Phosphate-mobilizing bacteria (PMB) play a critical role in the regulation of phosphorus availability in the soil. The microbial genes pqqC and phoD encode pyrroloquinoline quinone synthase and bacterial alkaline phosphatase, respectively, which regulate inorganic and organic phosphorus mobilization, and are therefore used as PMB markers. We examined the effects of soil properties in three Moso bamboo forest sites on the PMB communities that were profiled using high-throughput sequencing. We observed differentiated responses of pqqC- and phoD-harbouring PMB communities to various soil conditions. There was significant variation among the sites in the diversity and structure of the phoD-harbouring community, which correlated with variation in phosphorus levels and non-capillary porosity; soil organic carbon and soil water content also affected the structure of the phoD-harbouring community. However, no significant difference in the diversity of pqqC-harbouring community was observed among different sites, while the structure of the pqqC-harbouring bacteria community was affected by soil organic carbon and soil total nitrogen, but not soil phosphorus levels. Overall, changes in soil conditions affected the phoD-harbouring community more than the pqqC-harbouring community. These findings provide a new insight to explore the effects of soil conditions on microbial communities that solubilize inorganic phosphate and mineralize organic phosphate.

SUBMITTER: Shi W

PROVIDER: S-EPMC9249317 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:IntroductionConsidering the rapid growth and high biomass productivity, Moso bamboo (Phyllostachys edulis) has high carbon (C) sequestration potential, and different management practices can strongly modify its C pools. Soil microorganisms play an important role in C turnover through dead plant and microbial biomass degradation. To date, little is known about how different management practices affect microbial carbohydrate-active enzymes (CAZymes) and their responses to dead biomass degradation.MethodsBased on metagenomics analysis, this study analyzed CAZymes in three comparable stands from each Moso bamboo plantation: undisturbed (M0), extensively managed (M1), and intensively managed (M2).ResultsThe results showed that the number of CAZymes encoding plant-derived component degradation was higher than that encoding microbe-derived component degradation. Compared with the M0, the CAZyme families encoding plant-derived cellulose were significantly (p < 0.05) high in M2 and significantly (p < 0.05) low in M1. For microbe-derived components, the abundance of CAZymes involved in the bacterial-derived peptidoglycan was higher than that in fungal-derived components (chitin and glucans). Furthermore, M2 significantly increased the fungal-derived chitin and bacterial-derived peptidoglycan compared to M0, whereas M1 significantly decreased the fungal-derived glucans and significantly increased the bacterial-derived peptidoglycan. Four bacterial phyla (Acidobacteria, Actinobacteria, Proteobacteria, and Chloroflexi) mainly contributed to the degradation of C sources from the plant and microbial biomass. Redundancy analysis (RDA) and mantel test suggested the abundance of CAZyme encoding genes for plant and microbial biomass degradation are significantly correlated with soil pH, total P, and available K. Least Squares Path Modeling (PLS-PM) showed that management practices indirectly affect the CAZyme encoding genes associated with plant and microbial biomass degradation by regulating the soil pH and nutrients (total N and P), respectively.DiscussionOur study established that M2 and M1 impact dead biomass decomposition and C turnover, contributing to decreased C accumulation and establishing that the bacterial community plays the main role in C turnover in bamboo plantations.

Project description:IntroductionMoso bamboo (Phyllostachys edulis (Carrière) J. Houz.), the most widely distributed economic bamboo species in southern China, can easily invade adjacent communities due to its clonal reproduction. However, there is little information on the effects of its establishment and expansion to adjacent forest soil communities, particularly in planted forests.MethodsWe investigated the relationships between soil properties and the microbial community during bamboo invasion under different slope directions (shady or sunny slope) and positions (bottom, middle, or top slope), in three typical stand types (bottom: pure moso bamboo, middle: mixed stands of moso bamboo and Masson pine (Pinus massoniana Lamb.), and top: pure Masson pine) in the Lijiang River Basin. This study aimed to explore the effects of key environmental factors on soil microbial composition, diversity, and abundance.Results and discussionThe results showed that the abundance of Acidobacteria bacterium and Acidobacteria bacterium 13_2_20CM_58_27, and Verrucomicrobia bacterium decreased as the slope increased (p < 0.05), whereas the abundance of Alphaproteobacteria bacterium, Actinobacteria bacterium, Trebonia kvetii, and Bradyrhizobium erythrophlei increased as the slope increased (p < 0.05). However, the difference of slope direction on microbial communities was not significant. The pH, organic matter (OM) and total phosphorus (TP) were the key soil environmental factors; most microorganisms (Betaproteobacteria bacterium, Candidatus Eisenbacteria bacterium, Betaproteobacteria bacterium SCGC_AG - 212 - J23, Gemmatimonadetes bacterium, Actinobacteria bacterium 13_2_20CM_2_66_6, and Myxococcaceae bacterium) showed a positive relationship with pH and a negative relationship with OM and TP. Slope position significantly affected OM, calcium (Ca), total nitrogen (TN), available phosphorus (AP), hydrolyzed nitrogen (HN), pH, and microbial abundance and composition. Slope direction significantly affected TP and magnesium (Mg). The structural equations also indicated that slope position had an effect on microbial composition, abundance, and diversity. Slope position was negatively correlated with pH (r = -0.333, p = 0.034) and positively correlated with OM (r = 0.728, p < 0.001), TN (r = 0.538, p < 0.001) and Ca (r = 0.672, p < 0.001); pH was positively correlated with microbial composition (r = 0.634, p < 0.001), abundance (r = 0.553, p < 0.001) and diversity (r = 0.412, p = 0.002), TN was positively correlated with microbial composition (r = 0.220, p = 0.014) and abundance (r = 0.206, p = 0.013), and Ca was negatively correlated with microbial composition (r = -0.358, p = 0.003) and abundance (r = -0.317, p = 0.003). Slope position can also influence microbial composition (r = 0.452, p < 0.001) directly. In addition, slope direction had an indirect effect on microbial diversity through total potassium (TK). Therefore, we proposed that the different variations in microbial community during bamboo invasion could be related to the influence of invasion on the soil properties at different invasion stages.

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Diverse responses of pqqC- and phoD-harbouring bacterial communities to variation in soil properties of Moso bamboo forests.

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