Optimized in vitro three-dimensional invasion assay for quantifying a wide range of cancer cell invasive behavior.
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ABSTRACT: We describe a three-dimensional (3D) in vitro assay for quantifying cancer cell invasion into a 3D microenvironment with defined biochemical and biophysical properties. Researchers can quantify invasion dynamics (e.g., cell motility and directionality) and examine morphological changes during invasion, using live-cell and confocal imaging techniques. Together, these advantages over existing in vitro invasion assays, such as transwell-based assays, provide researchers with a valuable tool to gain insight into the mechanisms regulating cancer cell invasion. For complete details on the use and execution of this protocol, please refer to Padilla-Rodriguez et al. (2018) and Watson et al. (2021).
SUBMITTER: Hill SM
PROVIDER: S-EPMC9256944 | biostudies-literature |
REPOSITORIES: biostudies-literature
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