Transcriptomics

Dataset Information

0

Validated prediction of pro-invasive growth factors using a transcriptome-wide invasion signature derived from a complex 3-D invasion assay


ABSTRACT: The invasion of activated fibroblasts represents a key pathomechanism in fibrotic diseases, carcinogenesis and metastasis. Here, invading fibroblasts contribute to fibrotic extracellular matrix (ECM) formation and the initiation, progression, or resistance of cancer, respectively. To construct a transcriptome-wide signature of fibroblast invasion, we used a multiplex phenotypic 3D invasion assay using murine lung fibroblasts. Microarray-based gene expression profiles of invading and non-invading fibroblasts were highly distinct: 1049 genes were differentially regulated (>1.5-fold). An unbiased pathway analysis (Ingenuity) identified a significant enrichment for the functional clusters ‘invasion of cells’, ‘idiopathic pulmonary fibrosis (IPF)’ and ‘metastasis’. Particularly, matrix metalloprotease13 (MMP13), transforming growth factor (TGF)β1, Caveolin1 (Cav1), Phosphatase and Tensin Homolog (Pten), and secreted frizzled-related protein1 (Sfrp1) were among the highest regulated genes. In silico analysis by Ingenuity predicted TGFβ1, epidermal growth factor (EGF), fibroblast growth factor2 (FGF2), and platelet-derived growth factor (PDGF)-BB to induce invasion. As such, these growth factors were tested in the 3D invasion assay and displayed a significant induction of invasion, thus validating the transcriptome profile. Accordingly, our transcriptomic invasion signature describes the invading fibroblast phenotype in unprecedented detail and provides a tool for future functional studies of cell invasion and therapeutic modulation thereof.

ORGANISM(S): Mus musculus

PROVIDER: GSE55322 | GEO | 2015/10/05

SECONDARY ACCESSION(S): PRJNA239320

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-05-04 | PXD016882 | Pride
2010-07-08 | E-GEOD-21293 | biostudies-arrayexpress
2010-12-06 | E-GEOD-19426 | biostudies-arrayexpress
2023-11-08 | PXD046778 | Pride
2013-12-01 | E-GEOD-44458 | biostudies-arrayexpress
2013-08-05 | E-GEOD-40839 | biostudies-arrayexpress
2023-11-14 | GSE207561 | GEO
2021-02-24 | GSE167339 | GEO
2016-12-22 | GSE74057 | GEO
2016-12-22 | GSE74056 | GEO