Ontology highlight
ABSTRACT:
SUBMITTER: Sun L
PROVIDER: S-EPMC92690 | biostudies-literature | 2001 Mar
REPOSITORIES: biostudies-literature
Sun L L Ghosh I I Paulus H H Xu M Q MQ
Applied and environmental microbiology 20010301 3
Protein splicing in trans has been demonstrated both in vivo and in vitro by biochemical and immunological analyses, but in vivo production of a functional protein by trans-splicing has not been reported previously. In this study, we used the DnaE intein from Synechocystis sp. strain PCC6803, which presumably reconstitutes functional DnaE protein by trans-splicing in vivo, to produce functional herbicide-resistant acetolactate synthase II (ALSII) from two unlinked gene fragments in Escherichia c ...[more]