Ontology highlight
ABSTRACT:
SUBMITTER: Demichev V
PROVIDER: S-EPMC9270362 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Demichev Vadim V Szyrwiel Lukasz L Yu Fengchao F Teo Guo Ci GC Rosenberger George G Niewienda Agathe A Ludwig Daniela D Decker Jens J Kaspar-Schoenefeld Stephanie S Lilley Kathryn S KS Mülleder Michael M Nesvizhskii Alexey I AI Ralser Markus M
Nature communications 20220708 1
The dia-PASEF technology uses ion mobility separation to reduce signal interferences and increase sensitivity in proteomic experiments. Here we present a two-dimensional peak-picking algorithm and generation of optimized spectral libraries, as well as take advantage of neural network-based processing of dia-PASEF data. Our computational platform boosts proteomic depth by up to 83% compared to previous work, and is specifically beneficial for fast proteomic experiments and those with low sample a ...[more]