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Development of Rapid and Facile Solid-Phase Synthesis of PROTACs via a Variety of Binding Styles.

ABSTRACT: Optimizing linker design is important for ensuring efficient degradation activity of proteolysis-targeting chimeras (PROTACs). Therefore, developing a straightforward synthetic approach that combines the protein-of-interest ligand (POI ligand) and the ligand for E3 ubiquitin ligase (E3 ligand) in various binding styles through a linker is essential for rapid PROTAC syntheses. Herein, a solid-phase approach for convenient PROTAC synthesis is presented. We designed azide intermediates with different linker lengths to which the E3 ligand, pomalidomide, is attached and performed facile PROTACs synthesis by forming triazole, amide, and urea bonds from the intermediates.

SUBMITTER: Xu H

PROVIDER: S-EPMC9278092 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Development of Rapid and Facile Solid-Phase Synthesis of PROTACs via a Variety of Binding Styles.

Xu Hanqiao H Kurohara Takashi T Takano Reina R Yokoo Hidetomo H Shibata Norihito N Ohoka Nobumichi N Inoue Takao T Naito Mikihiko M Demizu Yosuke Y

ChemistryOpen 20220701 7

Optimizing linker design is important for ensuring efficient degradation activity of proteolysis-targeting chimeras (PROTACs). Therefore, developing a straightforward synthetic approach that combines the protein-of-interest ligand (POI ligand) and the ligand for E3 ubiquitin ligase (E3 ligand) in various binding styles through a linker is essential for rapid PROTAC syntheses. Herein, a solid-phase approach for convenient PROTAC synthesis is presented. We designed azide intermediates with differe ...[more]

PMID: 35822913

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