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A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons.

ABSTRACT: Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim. We report the engineering of a bright red fluorescent protein, Crimson, that enables long-term morphological labeling of neurons without aggregation or toxicity. Crimson is similar to mCherry and mKate2 in fluorescence spectra but is 100 and 28% greater in molecular brightness, respectively. We used a membrane-localized Crimson-CAAX to label thin neurites, dendritic spines and filopodia, enhancing detection of these small structures compared to cytosolic markers.

SUBMITTER: Ning L

PROVIDER: S-EPMC9278655 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons.

Ning Lin L Geng Yang Y Lovett-Barron Matthew M Niu Xiaoman X Deng Mengying M Wang Liang L Ataie Niloufar N Sens Alex A Ng Ho-Leung HL Chen Shoudeng S Deisseroth Karl K Lin Michael Z MZ Chu Jun J

Frontiers in cell and developmental biology 20220629

Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim. We report the engineering of a bright red fluorescent protein, Crimson, that enables long-term morphological labeling of neurons without aggregation or toxicity. Crimson is similar to mCherry and mKate2 in fluorescence spectra but is 100 and 28% ...[more]

PMID: 35846353

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