Dataset Information

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D₂ without improving overall vitamin D status: a randomized controlled trial.

ABSTRACT:

Purpose

This study investigated whether UVB-exposed wheat germ oil (WGO) is capable to improving the vitamin D status in healthy volunteers.

Methods

A randomized controlled human-intervention trial in parallel design was conducted in Jena (Germany) between February and April. Ultimately, 46 healthy males and females with low mean 25-hydroxyvitamin D (25(OH)D) levels (34.9 ± 10.6 nmol/L) were randomized into three groups receiving either no WGO oil (control, n = 14), 10 g non-exposed WGO per day (- UVB WGO, n = 16) or 10 g WGO, which was exposed for 10 min to ultraviolet B-light (UVB, intensity 500-630 µW/cm²) and provided 23.7 µg vitamin D (22.9 µg vitamin D₂ and 0.89 µg vitamin D₃) (+ UVB WGO, n = 16) for 6 weeks. Blood was obtained at baseline, after 3 and 6 weeks and analyzed for serum vitamin D-metabolite concentrations via LC-MS/MS.

Results

Participants who received the UVB-exposed WGO were characterized by an increase of circulating 25(OH)D₂ after 3 and 6 weeks of intervention. However, the 25(OH)D₃ concentrations decreased in the + UVB WGO group, while they increased in the control groups. Finally, the total 25(OH)D concentration (25(OH)D₂ + 25(OH)D₃) in the + UVB WGO group was lower than that of the non-WGO receiving control group after 6 weeks of treatment. In contrast, circulating vitamin D (vitamin D₂ + vitamin D₃) was higher in the + UVB WGO group than in the control group receiving no WGO.

Conclusion

UVB-exposed WGO containing 23.7 µg vitamin D can increase 25(OH)D₂ levels but do no improve total serum levels of 25(OH)D of vitamin D-insufficient subjects.

Trial registration

ClinicalTrials.gov: NCT03499327 (registered, April 13, 2018).

SUBMITTER: Bailer AC

PROVIDER: S-EPMC9279215 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D<sub>2</sub> without improving overall vitamin D status: a randomized controlled trial.

Bailer Anja C AC Philipp Sophie S Staudt Shabnam S Weidauer Thomas T Kiehntopf Michael M Lorkowski Stefan S Stangl Gabriele I GI Dawczynski Christine C

European journal of nutrition 20220227 5

<h4>Purpose</h4>This study investigated whether UVB-exposed wheat germ oil (WGO) is capable to improving the vitamin D status in healthy volunteers.<h4>Methods</h4>A randomized controlled human-intervention trial in parallel design was conducted in Jena (Germany) between February and April. Ultimately, 46 healthy males and females with low mean 25-hydroxyvitamin D (25(OH)D) levels (34.9 ± 10.6 nmol/L) were randomized into three groups receiving either no WGO oil (control, n = 14), 10 g non-expos ...[more]

PMID: 35220442

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Project description:A study was conducted to understand the effects of 25-hydroxyvitamin D3 (25OHD) and 1,25-dihydroxyvitamin D3 (1,25OHD) administration on the expression of key genes related to osteogenesis, adipogenesis, myogenesis, and vitamin D3 metabolism in the chicken embryo. A total of 120 fertilized Cobb 500 eggs were used in the current study and were reared under standard incubation conditions. On embryonic day 3 (ED 3), PBS (C), PBS with 40ng 1,25OHD (1,25D-L), 200ng 1,25OHD (1,25D-H), 40ng 25OHD (25D-L), or 200ng 25OHD (25D-H) were injected into the dorsal vein of developing embryos. Whole embryos were harvested at 1, 3, and 6h post-injection for gene expression analyses (n=8). Gene expression for key osteogenesis markers (RUNX2: runt-related transcription factor 2; BMP2: bone morphogenetic protein 2; COL1A2: collagen type I alpha 2 chain; BGLAP: bone gamma-carboxyglutamate protein; SPP1: secreted phosphoprotein 1; and ALP: alkaline phosphatese), adipogenesis markers (PPAR-γ: peroxisome proliferator-activated receptor gamma; FASN: fatty acid synthase; and FABP4: fatty acid binding protein 4), myogenesis markers (MYOG: myogenin; MYOD1: myogenic differentiation 1; and MYF5: myogenic factor 5), and the enzyme responsible for vitamin D3 inactivation (CYP24A1: cytochrome P450 family 24 subfamily A member 1) were measured using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Data were normalized by the ΔΔCT method and analyzed using a one-way ANOVA. Results indicated that at 1h post-injection, no differences were found among treatments. At 3h, the early osteogenesis differentiation marker, ALP, was increased by 1,25D-H and 25D-H, and 25D-H also stimulated the expression of adipogenesis markers (FAPB4 and FASN). In contrast, the expression of myogenesis markers (MYOD1 and MYF5) was suppressed by 25OHD or 1,25OHD treatments, respectively. At 6h, a late osteogenic differentiation marker, SPP1, was increased by 25D-H. MYOD1 and MYF5 were continuously suppressed by 25OHD treatments or 1,25D-H. The evidence of vitamin D3 metabolite retention was assessed by measuring CYP24A1 expression. At 1h, there were no differences in CYP24A1 expression. At 3h, all treatments upregulated CYP24A1 expression relative to control (PBS) embryos. However, at 6h, only the 25D-H group retained higher CYP24A1 expression compared to the other treatments. In conclusion, the results suggested both 1,25OHD and 25OHD induced chicken embryo osteogenesis and adipogenesis, but inhibited myogenesis during early chicken embryo development. The higher dosage of 25OHD showed a possibility of a longer retention time in the embryos.

Dataset Information

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D₂ without improving overall vitamin D status: a randomized controlled trial.

Purpose

Methods

Results

Conclusion

Trial registration

Publications

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D<sub>2</sub> without improving overall vitamin D status: a randomized controlled trial.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D2 without improving overall vitamin D status: a randomized controlled trial.

Purpose

Methods

Results

Conclusion

Trial registration

Publications

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D<sub>2</sub> without improving overall vitamin D status: a randomized controlled trial.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

UVB-exposed wheat germ oil increases serum 25-hydroxyvitamin D₂ without improving overall vitamin D status: a randomized controlled trial.