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Why do mice squeak? Toward a better understanding of defensive vocalization

ABSTRACT: Summary Although mice mostly communicate in the ultrasonic range, they also emit audible calls. We demonstrate that mice selectively bred for high anxiety-related behavior (HAB) have a high disposition for emitting sonic calls when caught by the tail. The vocalization was unrelated to pain but sensitive to anxiolytics. As revealed by manganese-enhanced MRI, HAB mice displayed an increased tonic activity of the periaqueductal gray (PAG). Selective inhibition of the dorsolateral PAG not only reduced anxiety-like behavior but also completely abolished sonic vocalization. Calls were emitted at a fundamental frequency of 3.8 kHz, which falls into the hearing range of numerous predators. Indeed, playback of sonic vocalization attracted rats if associated with a stimulus mouse. If played back to HAB mice, sonic calls were repellent in the absence of a conspecific but attractive in their presence. Our data demonstrate that sonic vocalization attracts both predators and conspecifics depending on the context. Graphical abstract Highlights • Sonic vocalization in threatening situations is prominent in highly anxious mice• It coincides with increased neuronal activity within the periaqueductal gray (PAG)• Pharmacological inhibition of the PAG attenuates sonic vocalization• Sonic calls attract both rats and mice in the presence of a stimulus mouse Biological sciences; Neuroscience; Behavioral neuroscience; Systems neuroscience

SUBMITTER: Ruat J

PROVIDER: S-EPMC9283514 | biostudies-literature |

REPOSITORIES: biostudies-literature

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