Microvesicles released from activated CD4⁺ T cells alter microvascular endothelial cell function.

ABSTRACT:

Background

Microvesicles are vesicles shed by plasma membranes following cell activation and apoptosis. The role of lymphocyte-derived microvesicles in endothelial function remains poorly understood.

Methods

CD4⁺ T cells isolated from peripheral blood of healthy human donors were stimulated using anti-CD3/anti-CD28-coated beads. Proteomic profiling of microvesicles was performed using linear discriminant analysis (LDA) from activated T cells (MV.Act) and nonactivated T cells (MV.NAct). In addition, data processing analysis was performed using MaxQUANT workflow. Differentially expressed proteins found in MV.Act or MV.NAct samples with identification frequency = 100%, which were selected by both LDA (p < .01) and MaxQUANT (p < .01) workflows, were defined as "high-c

SUBMITTER: Vdovenko D 

PROVIDER: S-EPMC9287044 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature