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Thiazole-Formulated Azomethine Compound for Three-Way Detection of Mercury Ions in Aqueous Media and Application in Living Cells.

ABSTRACT: Heavy metal ions are extremely poisonous and cause long-term harm to living organisms. Among these ions, mercury is the most toxic metal and has no notorious purpose in the human body. In this regard, an elegant azomethine thiazole compound AM1 was synthesized, and it was found to be highly sensitive to three-way detection of mercury ions with detection limits of 0.1126 × 10^-9 M (FL) and 0.64 × 10^-6 M (UV-vis). AM1 highlighted the capability to detect mercury ions through the colorimetric method, the fluorometric method, and via the naked eye in three-way detection. In addition, the structure of AM1 was confirmed by single-crystal X-ray diffraction studies and crystallized in a monoclinic crystal system with a P21/c space group, and it shows numerous noncovalent interactions in the crystal packing. The high sensitivity of AM1 to Hg²⁺ ions was imputed to the quenching mechanism and was estimated by Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (¹H-NMR), high-resolution mass spectrometry (HRMS), ultraviolet-visible (UV-vis) absorbance, fluorescence (FL) emission, Job's plot, B-H plot, and DFT calculation. Naked eye color change of AM1 solution to yellow and turn-off FL by the addition of mercury ion is due to complex formation. In addition to mercury ions, the sensor displayed a new absorption peak at around 240 nm. Furthermore, an AM1-coated test strip is used as the solid support sensor, and real-time detection of Hg²⁺ ions in the HeLa cell line by fluorescence microscopy is performed.

SUBMITTER: Umabharathi PS

PROVIDER: S-EPMC9301703 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Industrial pollution by heavy metal ions such as Hg2+ and Ag? is a universal problem owing to the toxicity of heavy metals. In this study, a novel nano-adsorbent, i.e., poly-2-mercapto-1,3,4-thiadiazole (PTT), was synthesized and used to selectively adsorb mercury and silver ions from aqueous solutions. PTT nanoparticles were synthesized via chemical oxidative dehydrogenation polymerization under mild conditions. Oxidant species, medium, monomer concentration, oxidant/monomer molar ratio, and polymerization temperature were optimized to obtain optimum yields. The molecular structure and morphology of the nanoparticles were analyzed by ultraviolet-visible (UV-Vis), Fourier transform infrared (FT-IR), matrix-assisted laser desorption/ionization/time-of-flight (MALDI/TOF) mass and X-ray photoelectron (XPS) spectroscopies, wide-angle X-ray diffraction (WAXD), theoretical calculations and transmission electron microscopy (TEM), respectively. It was found that the polymerization of 2-mercapto-1,3,4-thiodiazole occurs through head-to-tail coupling between the S(2) and C(5) positions. The PTT nanoparticles having a peculiar synergic combination of four kinds of active groups, S?, ?SH, N?N, and =N? with a small particle size of 30?200 nm exhibit ultrarapid initial adsorption rates of 1500 mg(Hg)·g-1·h-1 and 5364 mg(Ag)·g-1·h-1 and high adsorption capacities of up to 186.9 mg(Hg)·g-1 and 193.1 mg(Ag)·g-1, becoming ultrafast chelate nanosorbents with high adsorption capacities. Kinetic study indicates that the adsorption of Hg2+ and Ag? follows the pseudo-second-order model, suggesting a chemical adsorption as the rate-limiting step during the adsorption process. The Hg2+ and Ag?-loaded PTT nanoparticles could be effectively regenerated with 0.1 mol·L-1 EDTA or 1 mol·L-1 HNO? without significantly losing their adsorption capacities even after five adsorption?desorption cycles. With these impressive properties, PTT nanoparticles are very promising materials in the fields of water-treatment and precious metals recovery.

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Thiazole-Formulated Azomethine Compound for Three-Way Detection of Mercury Ions in Aqueous Media and Application in Living Cells.

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