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A Naphthalimide‐Based Fluorescent Probe for the Detection and Imaging of Mercury Ions in Living Cells

ABSTRACT: Abstract The selective and efficient monitoring of mercury (Hg2+) contamination found in the environment and ecosystem has been carried out. Thus, a new 1,8‐naphthalimide‐based fluorescent probe NADP for the detection of Hg2+ based on a fluorescence enhancement strategy has been designed and synthesized. The NADP probe can detect Hg2+ with high selectivity and sensitivity and a low detection limit of 13 nm. The detection mechanism was based on a Hg2+‐triggered deprotection reaction, resulting in a dramatic change in fluorescence from colorless to green at physiological pH. Most importantly, biological investigation has shown that the NADP probe can be successfully applied to the monitoring of Hg2+ in living cells and zebrafish with low cytotoxicity. A fluorescent probe for detecting mercury ions was synthesized. By comparing the fluorescence signals before and after the reaction, the detection of mercury ions was successfully realized. The NADP probe has been applied to cells and zebrafish due to their high selectivity, low detection limit and low toxicity, indicating that NADP probes have great potential in the detection of mercury in vivo and in the environment.

SUBMITTER: Lei S

PROVIDER: S-EPMC8562314 | biostudies-literature |

REPOSITORIES: biostudies-literature

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