Unknown

Dataset Information

0

Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T.


ABSTRACT: The Lactococcus lactis temperate bacteriophage BK5-T is one of twelve type phages that define L. lactis phage species. This paper describes the nucleotide sequence and analysis of a 21-kbp region of the BK5-T genome and completes the nucleotide sequence of the genome of this phage. The 40,003-nucleotide linear genome encodes 63 open reading frames. Sequence runoff experiments showed that the cohesive ends of the BK5-T genome contained a 12-bp 3' single-stranded overhang with the sequence 5'-CACACACATAGG-3'. Two major BK5-T structural proteins, of approximately 30 and 20 kDa, were identified, and N-terminal sequence analysis determined that they were encoded by orf7 and orf12, respectively. A 169-bp fragment containing a 37-bp direct repeat and several smaller repeat sequences conferred resistance to BK5-T infection when introduced in trans to the host cell and is likely a part of the BK5-T origin of replication (ori).

SUBMITTER: Mahanivong C 

PROVIDER: S-EPMC93057 | biostudies-literature | 2001 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T.

Mahanivong C C   Boyce J D JD   Davidson B E BE   Hillier A J AJ  

Applied and environmental microbiology 20010801 8


The Lactococcus lactis temperate bacteriophage BK5-T is one of twelve type phages that define L. lactis phage species. This paper describes the nucleotide sequence and analysis of a 21-kbp region of the BK5-T genome and completes the nucleotide sequence of the genome of this phage. The 40,003-nucleotide linear genome encodes 63 open reading frames. Sequence runoff experiments showed that the cohesive ends of the BK5-T genome contained a 12-bp 3' single-stranded overhang with the sequence 5'-CACA  ...[more]

Similar Datasets

| S-EPMC526828 | biostudies-other
| S-EPMC522090 | biostudies-literature
| S-EPMC3519859 | biostudies-literature
| S-EPMC3869327 | biostudies-literature
| S-EPMC91272 | biostudies-literature
| S-EPMC3738903 | biostudies-literature
| S-EPMC3921932 | biostudies-literature
| S-EPMC6546414 | biostudies-literature
| S-EPMC6965574 | biostudies-literature
| S-EPMC8407701 | biostudies-literature