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Evolutionary origin and functional diversification of aminotransferases.

ABSTRACT: Aminotransferases (ATs) are pyridoxal 5'-phosphate-dependent enzymes that catalyze the transamination reactions between amino acid donor and keto acid acceptor substrates. Modern AT enzymes constitute ∼2% of all classified enzymatic activities, play central roles in nitrogen metabolism, and generate multitude of primary and secondary metabolites. ATs likely diverged into four distinct AT classes before the appearance of the last universal common ancestor and further expanded to a large and diverse enzyme family. Although the AT family underwent an extensive functional specialization, many AT enzymes retained considerable substrate promiscuity and multifunctionality because of their inherent mechanistic, structural, and functional constraints. This review summarizes the evolutionary history, diverse metabolic roles, reaction mechanisms, and structure-function relationships of the AT family enzymes, with a special emphasis on their substrate promiscuity and multifunctionality. Comprehensive characterization of AT substrate specificity is still needed to reveal their true metabolic functions in interconnecting various branches of the nitrogen metabolic network in different organisms.

SUBMITTER: Koper K

PROVIDER: S-EPMC9309667 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Evolutionary origin and functional diversification of aminotransferases.

Koper Kaan K Han Sang-Woo SW Pastor Delia Casas DC Yoshikuni Yasuo Y Maeda Hiroshi A HA

The Journal of biological chemistry 20220611 8

Aminotransferases (ATs) are pyridoxal 5'-phosphate-dependent enzymes that catalyze the transamination reactions between amino acid donor and keto acid acceptor substrates. Modern AT enzymes constitute ∼2% of all classified enzymatic activities, play central roles in nitrogen metabolism, and generate multitude of primary and secondary metabolites. ATs likely diverged into four distinct AT classes before the appearance of the last universal common ancestor and further expanded to a large and diver ...[more]

PMID: 35697072

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Dataset Information

Evolutionary origin and functional diversification of aminotransferases.

Publications

Evolutionary origin and functional diversification of aminotransferases.

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