A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editing
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ABSTRACT:
SUBMITTER: Karagyaur M
PROVIDER: S-EPMC9324666 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:A light-activated genome editing platform based on the release of enzymes from a plasmonic nanoparticle carrier when exposed to biocompatible near-infrared light pulses is described. The platform relies on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of copper which is attached to double-stranded nucleic acids self-assembled on the gold nanoparticle surface. A protein fusion of the Cre recombinase containing a TAT internalization peptide sequence to achieve endosomal localization is also employed. High-resolution gene knock-in of a red fluorescent reporter is observed using a commercial two-photon microscope. High-throughput irradiation is described to generate useful quantities of edited cells.
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