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Identification of Nematicidal Metabolites from Purpureocillium lavendulum.


ABSTRACT: Purpureocillium lavendulum is a fungus with promising biocontrol applications. Here, transcriptome data acquired during the infection of Caenorhabditis elegans by Purpureocillium lavendulum showed that the transcription of metabolite synthesis genes was significantly up-regulated after 24 and 48 h of the fungus-nematode interaction. Then, the up-regulated transcription level of lipoxygenase was confirmed by RT-qPCR. The ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis of differential metabolites revealed that this interaction resulted in the emergence of new metabolites or enhanced the production of metabolites. The results of the UPLC-MS analysis and the nematicidal assay were used to establish optimal culturing conditions under which 12 metabolites, including 3 hydroxylated C18 fatty acids and 9 steroids, were isolated and identified. Among them, hydroxylated fatty acids showed pronounced nematicidal activity against Meloidogyne incognita, and two degradative sterols showed chemotaxis activity to M. incognita. This study lays a foundation for the function of lipoxygenase and its products during the infection of Purpureocillium lavendulum.

SUBMITTER: Liu R 

PROVIDER: S-EPMC9325011 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Identification of Nematicidal Metabolites from <i>Purpureocillium lavendulum</i>.

Liu Rui R   Bao Zheng-Xue ZX   Li Guo-Hong GH   Li Chun-Qiang CQ   Wang Shao-Lin SL   Pan Xue-Rong XR   Zhang Ke-Qin KQ   Zhao Pei-Ji PJ  

Microorganisms 20220702 7


<i>Purpureocillium lavendulum</i> is a fungus with promising biocontrol applications. Here, transcriptome data acquired during the infection of <i>Caenorhabditis elegans</i> by <i>Purpureocillium lavendulum</i> showed that the transcription of metabolite synthesis genes was significantly up-regulated after 24 and 48 h of the fungus-nematode interaction. Then, the up-regulated transcription level of lipoxygenase was confirmed by RT-qPCR. The ultra-performance liquid chromatography-mass spectromet  ...[more]

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