Droplet Microfluidic Device for Chemoenzymatic Sensing
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ABSTRACT: The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN–oxidoreductase) with substrates required for the reaction. The developed chip generates “water-in-oil” emulsion droplets with a volume of 0.1
SUBMITTER: Yakimov A
PROVIDER: S-EPMC9325247 | biostudies-literature |
REPOSITORIES: biostudies-literature
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