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Droplet Microfluidic Device for Chemoenzymatic Sensing


ABSTRACT: The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN–oxidoreductase) with substrates required for the reaction. The developed chip generates “water-in-oil” emulsion droplets with a volume of 0.1

SUBMITTER: Yakimov A 

PROVIDER: S-EPMC9325247 | biostudies-literature |

REPOSITORIES: biostudies-literature

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2015-07-21 | GSE65516 | GEO