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DGLYAT modulates Gadd45-mediated JNK activation and cell invasion.

ABSTRACT:

Background

Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion.

Results

We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and cell invasion. Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. Finally, Gadd45 knockdown suppressed scrib depletion-induced JNK pathway activation and cell invasion.

Conclusions

These evidences reveal the role of dGLYAT and Gadd45 in JNK-dependent cell invasion, and provide insight for the roles of their human homologs in cancers.

SUBMITTER: Xu M

PROVIDER: S-EPMC9357319 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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dGLYAT modulates Gadd45-mediated JNK activation and cell invasion.

Xu Meng M Ren Pu P Tian Juhui J Xiao Lisha L Hu Ping P Chen Ping P Li Wenzhe W Xue Lei L

Cell division 20220806 1

<h4>Background</h4>Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion.<h4>Results</h4>We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and cell invasion. Firstly, loss of dGLYAT suppressed scrib deplet ...[more]

PMID: 35933447

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