Dataset Information

A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.

ABSTRACT: The performance of commonly used assays for diagnosis of syphilis varies considerably depending on stage of infection and sample type. In response to the need for improved syphilis diagnostics, we develop assays that pair PCR pre-amplification of the tpp47 gene of Treponema pallidum subsp. pallidum with CRISPR-LwCas13a. The PCR-LwCas13a assay achieves an order of magnitude better analytical sensitivity than real-time PCR with equivalent specificity. When applied to a panel of 216 biological specimens, including 135 clinically confirmed primary and secondary syphilis samples, the PCR-LwCas13a assay demonstrates 93.3% clinical sensitivity and 100% specificity, outperforming tpp47 real-time PCR and rabbit-infectivity testing. We further adapt this approach to distinguish Treponema pallidum subsp. pallidum lineages and identify genetic markers of macrolide resistance. Our study demonstrates the potential of CRISPR-based approaches to improve diagnosis and epidemiological surveillance of syphilis.

SUBMITTER: Chen W

PROVIDER: S-EPMC9362966 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.

Chen Wentao W Luo Hao H Zeng Lihong L Pan Yuying Y Parr Jonathan B JB Jiang Yinbo Y Cunningham Clark H CH Hawley Kelly L KL Radolf Justin D JD Ke Wujian W Ou Jiangli J Yang Jianjiang J Yang Bin B Zheng Heping H

Nature communications 20220809 1

The performance of commonly used assays for diagnosis of syphilis varies considerably depending on stage of infection and sample type. In response to the need for improved syphilis diagnostics, we develop assays that pair PCR pre-amplification of the tpp47 gene of Treponema pallidum subsp. pallidum with CRISPR-LwCas13a. The PCR-LwCas13a assay achieves an order of magnitude better analytical sensitivity than real-time PCR with equivalent specificity. When applied to a panel of 216 biological spec ...[more]

PMID: 35945210

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Dataset Information

A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.

Publications

A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples.

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