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Preanalytical Pitfalls in Untargeted Plasma Nuclear Magnetic Resonance Metabolomics of Endocrine Hypertension.

ABSTRACT: Despite considerable morbidity and mortality, numerous cases of endocrine hypertension (EHT) forms, including primary aldosteronism (PA), pheochromocytoma and functional paraganglioma (PPGL), and Cushing's syndrome (CS), remain undetected. We aimed to establish signatures for the different forms of EHT, investigate potentially confounding effects and establish unbiased disease biomarkers. Plasma samples were obtained from 13 biobanks across seven countries and analyzed using untargeted NMR metabolomics. We compared unstratified samples of 106 PHT patients to 231 EHT patients, including 104 PA, 94 PPGL and 33 CS patients. Spectra were subjected to a multivariate statistical comparison of PHT to EHT forms and the associated signatures were obtained. Three approaches were applied to investigate and correct confounding effects. Though we found signatures that could separate PHT from EHT forms, there were also key similarities with the signatures of sample center of origin and sample age. The study design restricted the applicability of the corrections employed. With the samples that were available, no biomarkers for PHT vs. EHT could be identified. The complexity of the confounding effects, evidenced by their robustness to correction approaches, highlighted the need for a consensus on how to deal with variabilities probably attributed to preanalytical factors in retrospective, multicenter metabolomics studies.

SUBMITTER: Bliziotis NG

PROVIDER: S-EPMC9394285 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Preanalytical Pitfalls in Untargeted Plasma Nuclear Magnetic Resonance Metabolomics of Endocrine Hypertension.

Bliziotis Nikolaos G NG Kluijtmans Leo A J LAJ Tinnevelt Gerjen H GH Reel Parminder P Reel Smarti S Langton Katharina K Robledo Mercedes M Pamporaki Christina C Pecori Alessio A Van Kralingen Josie J Tetti Martina M Engelke Udo F H UFH Erlic Zoran Z Engel Jasper J Deutschbein Timo T Nölting Svenja S Prejbisz Aleksander A Richter Susan S Adamski Jerzy J Januszewicz Andrzej A Ceccato Filippo F Scaroni Carla C Dennedy Michael C MC Williams Tracy A TA Lenzini Livia L Gimenez-Roqueplo Anne-Paule AP Davies Eleanor E Fassnacht Martin M Remde Hanna H Eisenhofer Graeme G Beuschlein Felix F Kroiss Matthias M Jefferson Emily E Zennaro Maria-Christina MC Wevers Ron A RA Jansen Jeroen J JJ Deinum Jaap J Timmers Henri J L M HJLM

Metabolites 20220724 8

Despite considerable morbidity and mortality, numerous cases of endocrine hypertension (EHT) forms, including primary aldosteronism (PA), pheochromocytoma and functional paraganglioma (PPGL), and Cushing's syndrome (CS), remain undetected. We aimed to establish signatures for the different forms of EHT, investigate potentially confounding effects and establish unbiased disease biomarkers. Plasma samples were obtained from 13 biobanks across seven countries and analyzed using untargeted NMR metab ...[more]

PMID: 35893246

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Dataset Information

Preanalytical Pitfalls in Untargeted Plasma Nuclear Magnetic Resonance Metabolomics of Endocrine Hypertension.

Publications

Preanalytical Pitfalls in Untargeted Plasma Nuclear Magnetic Resonance Metabolomics of Endocrine Hypertension.

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