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The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells.
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ABSTRACT: Background
Recently, various regenerative therapies have been developed based on induced pluripotent stem (iPS) cells. However, hygienic control strategies have not been established at the manufacturing facilities. We aimed to evaluate the safety and effects of continuous exposure to low-dose chlorine dioxide (ClO2) gas on cell fates, and to determine the optimum dose for safe usage of this disinfectant.Methods
We cultured an iPS cell line in the absence or presence of various doses of ClO2 gas. We evaluated cell proliferation, cell death, the maintenance of undifferentiated state, and cell senescence.Results
We found that iPS cell proliferation was not affected by 0.05 or 0.1 ppmv ClO2 gas in the atmosphere. Although 0.1 ppmv ClO2 slightly affected apoptosis, it was not a significant effect. Moreover, neither at 0.05 nor 0.1 ppmv ClO2 gas significantly affected the characteristics of iPS cells.Discussion and conclusion
Continuous exposure to 0.05 or 0.1 ppmv ClO2 gas did not affect the fate of iPS cells. These results may contribute to the development of new strategies for hygiene control in cell processing facilities.
SUBMITTER: Okawa R
PROVIDER: S-EPMC9420961 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
The effects of continuous exposure to low-dose chlorine dioxide gas on the characteristics of induced pluripotent stem cells.
Regenerative therapy 20220823
<h4>Background</h4>Recently, various regenerative therapies have been developed based on induced pluripotent stem (iPS) cells. However, hygienic control strategies have not been established at the manufacturing facilities. We aimed to evaluate the safety and effects of continuous exposure to low-dose chlorine dioxide (ClO<sub>2</sub>) gas on cell fates, and to determine the optimum dose for safe usage of this disinfectant.<h4>Methods</h4>We cultured an iPS cell line in the absence or presence of ...[more]
