Unknown

Dataset Information

0

Pig Coat Color Manipulation by MC1R Gene Editing.


ABSTRACT: Black coat color in pigs is determined by the dominant E allele at the MC1R locus. Through comparing MC1R gene sequences between recessive e and dominant ED1 alleles, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis. With the aim of devising a genetic modification method for pig coat color manipulation, we mutated the e allele in the Duroc breed to the dominant ED1 allele using CRISPR-mediated homologous recombination for the four mutation substitutions at the MC1R locus. The MC1R-modified Duroc pigs generated using the allele replacement strategy displayed uniform black coat color across the body. A genotyping assay showed that the MC1R-modified Duroc pigs had a heterozygous ED1/e allele at the MC1R locus; in addition, the pigs remained in the Duroc genetic background. Our work offers a gene editing method for pig coat color manipulation, which could value the culture of new pig varieties meeting the needs of diversified market.

SUBMITTER: Zhong H 

PROVIDER: S-EPMC9499681 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Pig Coat Color Manipulation by <i>MC1R</i> Gene Editing.

Zhong Haiwen H   Zhang Jian J   Tan Cheng C   Shi Junsong J   Yang Jie J   Cai Gengyuan G   Wu Zhenfang Z   Yang Huaqiang H  

International journal of molecular sciences 20220908 18


Black coat color in pigs is determined by the dominant <i>E</i> allele at the <i>MC1R</i> locus. Through comparing <i>MC1R</i> gene sequences between recessive <i>e</i> and dominant <i>E<sup>D1</sup></i> alleles, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis. With the aim of devising a genetic modification method for pig coat color manipulation, we mutated the <i>e</i> allele in the Duroc breed to the dominant <i>E<sup>D1</sup></i> allele u  ...[more]

Similar Datasets

| S-EPMC3776380 | biostudies-literature
| S-EPMC1460407 | biostudies-other
| S-EPMC6511826 | biostudies-literature
| S-EPMC4885240 | biostudies-literature
| S-EPMC6447268 | biostudies-literature
| S-EPMC9031378 | biostudies-literature
| S-EPMC2936272 | biostudies-literature
| S-EPMC6759607 | biostudies-literature
| S-EPMC6108395 | biostudies-literature
| S-EPMC3924621 | biostudies-literature