DIA label-free proteomic analysis of murine bone-marrow-derived macrophages.
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ABSTRACT:
SUBMITTER: Baker CP
PROVIDER: S-EPMC9519785 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
DIA label-free proteomic analysis of murine bone-marrow-derived macrophages.
Baker Christa P CP Phair Iain R IR Brenes Alejandro J AJ Atrih Abdelmadjid A Ryan Dylan G DG Bruderer Roland R Dinkova-Kostova Albena T AT Lamont Douglas J DJ Arthur J Simon C JSC Howden Andrew J M AJM
STAR protocols 20220926 4
Here, we describe an optimized protocol to analyze murine bone-marrow-derived macrophages using label-free data-independent acquisition (DIA) proteomics. We provide a complete step-by-step protocol describing sample preparation utilizing the S-Trap approach for on-column digestion and peptide purification. We then detail mass spectrometry data acquisition and approaches for data analysis. Single-shot DIA protocols achieve comparable proteomic depth with data-dependent MS approaches without the n ...[more]
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