Dataset Information

A method for the mix design of low carbon concrete towards industrial production.

ABSTRACT: The introduction of newly developed blended cements into the mass market is essential to ensure an effective reduction of the carbon footprint related to cement production. To facilitate this process, formulating mix proportions using pastes and/or mortars rather than concrete can be a great advantage. However, for the upscaling towards industrial concrete it is then essential to maintain the target rheological and mechanical properties, something that is all too often challenging. In this work, a procedure facilitating such an upscaling was illustrated in the form of a flow chart. Specifically, best practices to obtain a good correlation between concrete prepared in a laboratory and one prepared in a plant were presented. This includes new data showing how to accommodate for possible differences in temperature and/or water content between both situations. The dataset of state-of-the-art correlations between mechanical performance and heat of hydration, considering w/b ratios relevant to practice, were expanded. This greatly facilitates the mix design of concrete with particularly low clinker contents, which in this work were illustrated with a blended cement containing only 50% clinker.

Supplementary information

The online version contains supplementary material available at 10.1617/s11527-022-02040-5.

SUBMITTER: Boscaro F

PROVIDER: S-EPMC9522730 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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A method for the mix design of low carbon concrete towards industrial production.

Boscaro Federica F Flatt Robert J RJ

Materials and structures 20220929 8

The introduction of newly developed blended cements into the mass market is essential to ensure an effective reduction of the carbon footprint related to cement production. To facilitate this process, formulating mix proportions using pastes and/or mortars rather than concrete can be a great advantage. However, for the upscaling towards industrial concrete it is then essential to maintain the target rheological and mechanical properties, something that is all too often challenging. In this work, ...[more]

PMID: 36193284

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Project description:BackgroundSugarcane hemicellulosic material is a compelling source of usually neglected xylose that could figure as feedstock to produce chemical building blocks of high economic value, such as xylitol. In this context, Saccharomyces cerevisiae strains typically used in the Brazilian bioethanol industry are a robust chassis for genetic engineering, given their robustness towards harsh operational conditions and outstanding fermentation performance. Nevertheless, there are no reports on the use of these strains for xylitol production using sugarcane hydrolysate.ResultsPotential single-guided RNA off-targets were analyzed in two preeminent industrial strains (PE-2 and SA-1), providing a database of 5'-NGG 20 nucleotide sequences and guidelines for the fast and cost-effective CRISPR editing of such strains. After genomic integration of a NADPH-preferring xylose reductase (XR), FMYX (SA-1 hoΔ::xyl1) and CENPKX (CEN.PK-122 hoΔ::xyl1) were tested in varying cultivation conditions for xylitol productivity to infer influence of the genetic background. Near-theoretical yields were achieved for all strains; however, the industrial consistently outperformed the laboratory strain. Batch fermentation of raw sugarcane straw hydrolysate with remaining solid particles represented a challenge for xylose metabolization, and 3.65 ± 0.16 g/L xylitol titer was achieved by FMYX. Finally, quantification of NADPH - cofactor implied in XR activity - revealed that FMYX has 33% more available cofactors than CENPKX.ConclusionsAlthough widely used in several S. cerevisiae strains, this is the first report of CRISPR-Cas9 editing major yeast of the Brazilian bioethanol industry. Fermentative assays of xylose consumption revealed that NADPH availability is closely related to mutant strains' performance. We also pioneer the use of sugarcane straw as a substrate for xylitol production. Finally, we demonstrate how industrial background SA-1 is a compelling chassis for the second-generation industry, given its inhibitor tolerance and better redox environment that may favor production of reduced sugars.

Dataset Information

A method for the mix design of low carbon concrete towards industrial production.

Supplementary information

Publications

A method for the mix design of low carbon concrete towards industrial production.

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