Effect of Nickel Ions on the Physiological and Transcriptional Responses to Carbon and Nitrogen Metabolism in Tomato Roots under Low Nitrogen Levels.

ABSTRACT: Nickel (Ni) is an essential trace element for plant growth and a component of the plant body that has many different functions in plants. Although it has been confirmed that nickel ions (Ni²⁺) havea certain regulatory effect on nitrogen (N) metabolism, there are not enough data to prove whether exogenous Ni²⁺ can increase the carbon (C) and N metabolism in the roots of tomato seedlingsunder low-nitrogen (LN) conditions. Therefore, through the present experiment, we revealed the key mechanism of Ni²⁺-mediated tomato root tolerance to LN levels. Tomato plants were cultured at two different N levels (7.66 and 0.383 mmol L^-1) and two different Ni²⁺ levels (0 and 0.1 mg L^-1 NiSO₄ 6H₂O) under hydroponic conditions. After nine days, we collected roots for physiological, biochemical, and transcriptome sequencing analyses and found that the activities of N assimilation-related enzymes decreased at LN levels. In contrast, Ni²⁺ significantly increased the activities of N assimilation-related enzymes and increased the contents of nitrate (NO₃^-), ammonium (NH₄⁺), and total amino acids. Through root transcriptomic analysis, 3738 differentially expressed genes (DEGs) were identified. DEGs related to C and N metabolism were downregulated after LN application. However, after Ni²⁺ treatment, PK, PDHB, GAPDH, NR, NiR, GS, GOGAT, and other DEGs related to C and N metabolism were significantly upregulated. In conclusion, our results suggest that Ni²⁺ can regulate the C and N metabolism pathways in tomato roots to alleviate the impact of LN levels.

SUBMITTER: Zhang K 

PROVIDER: S-EPMC9569439 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature