Dataset Information

Quantitative spatial mapping of tissue water and lipid content using spatial frequency domain imaging in the 900- to 1000-nm wavelength region.

ABSTRACT:

Significance

Water and lipid are key participants of many biological processes, but there are few label-free, non-contact optical methods that can spatially map these components in-vivo. Shortwave infrared meso-patterned imaging (SWIR-MPI) is an emerging technique that successfully addresses this need. However, it requires a dedicated SWIR camera to probe the 900- to 1300-nm wavelength region, which hinders practical translation of the technology.

Aim

Compared with SWIR-MPI, we aim to develop a new technique that can dramatically reduce the cost in detector while maintaining high accuracy for the quantification of tissue water and lipid content.

Approach

By utilizing water and lipid absorption features in the 900- to 1000-nm wavelength region as well as optimal wavelength and spatial frequency combinations, we develop a new imaging technique based on spatial frequency domain imaging to quantitatively map tissue water and lipid content using a regular silicon-based camera.

Results

The proposed method is validated with a phantom study, which shows average error of 0.9 ± 1.2 % for water content estimation, and -0.4 ± 0.7 % for lipid content estimation, respectively. The proposed method is also demonstrated for ex vivo porcine tissue lipid mapping as well as in-vivo longitudinal water content monitoring.

Conclusions

The proposed technique enables spatial mapping of tissue water and lipid content with the cost in detector reduced by two orders of magnitude compared with SWIR-MPI while maintaining high accuracy. The experimental results highlight the potential of this technique for substantial impact in both scientific and industrial applications.

SUBMITTER: Song B

PROVIDER: S-EPMC9612091 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Publications

Quantitative spatial mapping of tissue water and lipid content using spatial frequency domain imaging in the 900- to 1000-nm wavelength region.

Song Bowen B Yin Xinman X Fan Yubo Y Zhao Yanyu Y

Journal of biomedical optics 20221001 10

<h4>Significance</h4>Water and lipid are key participants of many biological processes, but there are few label-free, non-contact optical methods that can spatially map these components in-vivo. Shortwave infrared meso-patterned imaging (SWIR-MPI) is an emerging technique that successfully addresses this need. However, it requires a dedicated SWIR camera to probe the 900- to 1300-nm wavelength region, which hinders practical translation of the technology.<h4>Aim</h4>Compared with SWIR-MPI, we ai ...[more]

PMID: 36303279

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Project description:We report the synthesis and properties of the first representative of a new class of PtL2 complexes with ambidentate mixed-donor cyanoxime ligands [L = 2-cyano-2-oximino-N,N'-diethylaminoacetamide, DECO (1)]. Three differently colored polymorphs of "Pt(DECO)2" (3-5) were isolated, with the first two being crystallographically characterized. The dark-green complex [Pt(DECO)2]n (5) spontaneously forms in aqueous solution via aggregation of yellow monomeric complex 3 into the red dimer [Pt(DECO)2]2 (4), followed by further oligomerization into coordination polymer 5. A spectroscopic and light-scattering study revealed a "poker-chips"-type 1D polymeric structure of 5 in which units are held by noncovalent metallophilic interactions, forming a Pt---Pt wire. The polymer 5 shows a broad absorption at 400-900 nm and emission at unusually long wavelengths in the range of 1000-1100 nm in the solid state. The near-infrared (NIR) emission of polymer 5 is due to the formation of a small amount of nonstoichiometric mixed-valence Pt(II)/Pt(IV) species during synthesis. A featureless electron paramagnetic resonance spectrum of solid sample 5 recorded at +23 and -193 °C evidences the absence of Pt(III) states, and the compound represents a "solid solution" containing mixed-valence Pt(II)/Pt(IV) centers. Exposure of KBr pellets with 5% 5 to Br2 vapors leads to an immediate ∼30% increase in the intensity of photoluminescence at 1024 nm, which confirms the role and importance of mixed-valence species for the NIR emission. Thus, the emission is further enhanced upon additional oxidation of Pt(II) centers, which improves delocalization of electrons along the Pt---Pt vector. Other polymorph of the "Pt(DECO)2" complex--monomer--did not demonstrate luminescent properties in solutions and the solid state. An excitation scan of 5 embedded in KBr tablets revealed an emission only weakly dependent on the wavelength of excitation. The NIR emission of quasi-1D complex 5 was studied in the range of -193 to +67 °C. Data showed a blue shift of λmax and a simultaneous increase in the emission line intensity with a temperature rise, which is explained by analogy with similar behavior of known quasi-1D K2[Pt(CN)4]-based solids, quantum dots, and quantum wells with delocalized carriers. The presented finding opens a route to a new class of platinum cyanoxime based NIR emissive complexes that could be used in the design of novel NIR emitters and imaging agents.

Dataset Information

Quantitative spatial mapping of tissue water and lipid content using spatial frequency domain imaging in the 900- to 1000-nm wavelength region.

Significance

Aim

Approach

Results

Conclusions

Publications

Quantitative spatial mapping of tissue water and lipid content using spatial frequency domain imaging in the 900- to 1000-nm wavelength region.

Similar Datasets

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