Dataset Information

Treatment of Liquid Digestate by Green Algal Isolates from Artificial Eutrophic Pond.

ABSTRACT: The ability of aquatic microalgae to treat the liquid digestate obtained from the anaerobic digestion of plant waste was investigated. Microalgae were isolated from natural environment for a laboratory-scale cultivation and were then used to remove nutrients and organic contaminants from the liquid digestate. It was shown that the microalgae consortia (Tetradesmus obliquus, Microglena sp., Desmodesmus subspicatus) could reduce nitrogen, phosphates, and total COD by up to 70%, 57%, and 95%, respectively. A new algae genus Microglena was isolated, which in a consortium with Tetradesmus obliquus and Desmodesmus subspicatus exhibited a high efficiency in the removal of both organic contaminants and nutrients from the liquid fraction of digestate.

SUBMITTER: Sobolewska E

PROVIDER: S-EPMC9612388 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Treatment of Liquid Digestate by Green Algal Isolates from Artificial Eutrophic Pond.

Sobolewska Ewelina E Borowski Sebastian S Nowicka-Krawczyk Paulina P Banach Katarzyna K

Molecules (Basel, Switzerland) 20221013 20

The ability of aquatic microalgae to treat the liquid digestate obtained from the anaerobic digestion of plant waste was investigated. Microalgae were isolated from natural environment for a laboratory-scale cultivation and were then used to remove nutrients and organic contaminants from the liquid digestate. It was shown that the microalgae consortia (Tetradesmus obliquus, Microglena sp., Desmodesmus subspicatus) could reduce nitrogen, phosphates, and total COD by up to 70% ...[more]

PMID: 36296449

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Project description:Wood bottom and fly ashes were added to the anaerobic digestate using sulfuric, hydrochloric, nitric, and lactic acids, as pH conditioners and sorption activating agents. Minimum (pH of zero-point charge), mild, and severe acidification of the samples were tested. The solid-liquid separation achieved was accounted visually and with the measurement of the masses of water-soluble extract and water-insoluble material isolated. The average mass of the blend was 36.61 ± 0.68 g, including the extractant agent that was prepared with the commercial acids and ultrapure milli-QⓇ water. During the 144-h incubation of the mixtures at 20 °C and 0.17 x g, the shares of the solid and liquid were determined by centrifugation of the destructive samples at 3,130.40 x g for 5 minutes and 3-µm filtration of the supernatant. Before weighing the water-insoluble material, both the pellet that remained in the tube and the filter cake were dried at 70 °C until reaching constant weight. There was a significant increase in the amount of water-insoluble phase of the wood bottom ash due to the activation with lactic and sulfuric acids. The treatment of the wood fly ash and the agrowaste digestate with the hydrochloric acid showed an increase in the formation of water-soluble extract, in direct relation with the acidic pH of the blend. The characterization of the pH of the WS extract was performed with a Mettler ToledoⓇ Seven CompactTM S220 pH/Ion meter. The conditions of this process can be further optimized and each of the fractions can be characterized, in terms of nutrient content, to confirm the efficiency of the separation. More complex and ambitious processes can be designed for combining the wood ash and the anaerobic digestate. The performance of this treatment involving wood ashes and commercial acids can be extrapolated to other type of organic manures with a moisture content of approximately 95%, to improve their management in terms of reducing the cost of storage and transportation for land application below £5 per tonne.

Project description:Geochemical analyses alongside molecular techniques were used to characterize the microbial ecology and biogeochemistry of an outdoor spent nuclear fuel storage pond at Sellafield, United Kingdom, that is susceptible to seasonal algal blooms that cause plant downtime. 18S rRNA gene profiling of the filtered biomass samples showed the increasing dominance of a species closely related to the alga Haematococcus pluvialis, alongside 16S rRNA genes affiliated with a diversity of freshwater bacteria, including Proteobacteria and Cyanobacteria High retention of 137Cs and 90Sr on pond water filters coincided with high levels of microbial biomass in the pond, suggesting that microbial colonization may have an important control on radionuclide fate in the pond. To interpret the unexpected dominance of Haematococcus species during bloom events in this extreme environment, the physiological response of H. pluvialis to environmentally relevant ionizing radiation doses was assessed. Irradiated laboratory cultures produced significant quantities of the antioxidant astaxanthin, consistent with pigmentation observed in pond samples. Fourier transform infrared (FT-IR) spectroscopy suggested that radiation did not have a widespread impact on the metabolic fingerprint of H. pluvialis in laboratory experiments, despite the 80-Gy dose. This study suggests that the production of astaxanthin-rich encysted cells may be related to the preservation of the Haematococcus phenotype, potentially allowing it to survive oxidative stress arising from radiation doses associated with the spent nuclear fuel. The oligotrophic and radiologically extreme conditions in this environment do not prevent extensive colonization by microbial communities, which play a defining role in controlling the biogeochemical fate of major radioactive species present.IMPORTANCE Spent nuclear fuel is stored underwater in large ponds prior to processing and disposal. Such environments are intensively radioactive but can be colonized by microorganisms. Colonization of such inhospitable radioactive ponds is surprising, and the survival mechanisms that microbes use is of fundamental interest. It is also important to study these unusual ecosystems, as microbes growing in the pond waters may accumulate radionuclides present in the waters (for bioremediation applications), while high cell loads can hamper management of the ponds due to poor visibility. In this study, an outdoor pond at the U.K. Sellafield facility was colonized by a seasonal bloom of microorganisms, able to accumulate high levels of 137Cs and 90Sr and dominated by the alga Haematococcus This organism is not normally associated with deep water bodies, but it can adapt to radioactive environments via the production of the pigment astaxanthin, which protects the cells from radiation damage.

Dataset Information

Treatment of Liquid Digestate by Green Algal Isolates from Artificial Eutrophic Pond.

Publications

Treatment of Liquid Digestate by Green Algal Isolates from Artificial Eutrophic Pond.

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