Ontology highlight
ABSTRACT:
SUBMITTER: Hubschmann V
PROVIDER: S-EPMC9678782 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Hübschmann Verena V Korkut-Demirbaş Medina M Siegert Sandra S
STAR protocols 20221118 4
To understand how potential gene manipulations affect in vitro microglia, we provide a set of short protocols to evaluate microglia identity and function. We detail steps for immunostaining to determine microglia identity. We describe three functional assays for microglia: phagocytosis, calcium response following ATP stimulation, and cytokine expression upon inflammatory stimuli. We apply these protocols to human induced-pluripotent-stem-cell (hiPSC)-derived microglia, but they can be also appli ...[more]