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Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples.

ABSTRACT: Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development. For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).

SUBMITTER: Pankaew S

PROVIDER: S-EPMC9698364 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples.

Pankaew Saran S Grosjean Clémence C Quessada Julie J Loosveld Marie M Potier Delphine D Payet-Bornet Dominique D Nozais Mathis M

STAR protocols 20211220 1

Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply m ...[more]

PMID: 36475567

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