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Ion selectivity mechanism of the MgtE channel for Mg2+ over Ca2.


ABSTRACT: MgtE is a Mg2+-selective ion channel whose orthologs are widely distributed from prokaryotes to eukaryotes, including humans, and are important participants in the maintenance of cellular Mg2+ homeostasis. The previous high-resolution structure determination of the MgtE transmembrane (TM) domain in complex with Mg2+ ions revealed a recognition mechanism of MgtE for Mg2+ ions. In contrast, the previous Ca2+-bound structure of the MgtE TM domain was determined only at moderate resolution (3.2 Å resolution), which was insufficient to visualize the water molecules coordinated to Ca2+ ions. Here, we showed that the metal-binding site of the MgtE TM domain binds to Mg2+ ∼500-fold more strongly than to Ca2+. We then determined the crystal structure of the MgtE TM domain in complex with Ca2+ ions at a higher resolution (2.5 Å resolution), revealing hexahydrated Ca2+. These results provide mechanistic insights into the ion selectivity of MgtE for Mg2+ over Ca2+.

SUBMITTER: Teng X 

PROVIDER: S-EPMC9708914 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Ion selectivity mechanism of the MgtE channel for Mg<sup>2+</sup> over Ca<sup>2</sup>.

Teng Xinyu X   Sheng Danqi D   Wang Jin J   Yu Ye Y   Hattori Motoyuki M  

iScience 20221113 12


MgtE is a Mg<sup>2+</sup>-selective ion channel whose orthologs are widely distributed from prokaryotes to eukaryotes, including humans, and are important participants in the maintenance of cellular Mg<sup>2+</sup> homeostasis. The previous high-resolution structure determination of the MgtE transmembrane (TM) domain in complex with Mg<sup>2+</sup> ions revealed a recognition mechanism of MgtE for Mg<sup>2+</sup> ions. In contrast, the previous Ca<sup>2+</sup>-bound structure of the MgtE TM doma  ...[more]

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