Project description:Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection. We then detail embryo dissociation followed by steps to pick, wash, and dispense cells in plates.

| S-EPMC10300299 | biostudies-literature

Systems biology approaches to identify developmental bases for lung diseases.

Project description:A greater understanding of the regulatory processes contributing to lung development could be helpful to identify strategies to ameliorate morbidity and mortality in premature infants and to identify individuals at risk for congenital and/or chronic lung diseases. Over the past decade, genomics technologies have enabled the production of rich gene expression databases providing information for all genes across developmental time or in diseased tissue. These data sets facilitate systems biology approaches for identifying underlying biological modules and programs contributing to the complex processes of normal development and those that may be associated with disease states. The next decade will undoubtedly see rapid and significant advances in redefining both lung development and disease at the systems level.

| S-EPMC3615902 | biostudies-literature

Editorial: Molecular biomarkers in the prediction, diagnosis, and prognosis of neurodegenerative diseases.

Project description: Not available

| S-EPMC10285512 | biostudies-literature

Editorial: Molecular Function and Regulation of Non-coding RNAs in Multifactorial Diseases.

Project description: Not available

| S-EPMC4760206 | biostudies-literature

Editorial: Developmental delay and intellectual disability.

Project description: Not available

| S-EPMC9493353 | biostudies-literature

RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo.

Project description:It is widely assumed that the key rate-limiting step in gene activation is the recruitment of RNA polymerase II (Pol II) to the core promoter. Although there are well-documented examples in which Pol II is recruited to a gene but stalls, a general role for Pol II stalling in development has not been established. We have carried out comprehensive Pol II chromatin immunoprecipitation microarray (ChIP-chip) assays in Drosophila embryos and identified three distinct Pol II binding behaviors: active (uniform binding across the entire transcription unit), no binding, and stalled (binding at the transcription start site). The notable feature of the approximately 10% genes that are stalled is that they are highly enriched for developmental control genes, which are either repressed or poised for activation during later stages of embryogenesis. We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development.

| S-EPMC2824921 | biostudies-literature

Editorial: Epigenetic Regulation in Cardiovascular Diseases.

Project description: Not available

| S-EPMC8787130 | biostudies-literature