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First Isolation of a Herpesvirus (Family Alloherpesviridae) from Great Lakes Lake Sturgeon (Acipenser fulvescens).

ABSTRACT: The lake sturgeon (Acipenser fulvescens; LST) is the only native sturgeon species in the Great Lakes (GL), but due to multiple factors, their current populations are estimated to be <1% of historical abundances. Little is known about infectious diseases affecting GL-LST in hatchery and wild settings. Therefore, a two-year disease surveillance study was undertaken, resulting in the detection and first in vitro isolation of a herpesvirus from grossly apparent cutaneous lesions in wild adult LST inhabiting two GL watersheds (Erie and Huron). Histological and ultrastructural examination of lesions revealed proliferative epidermitis associated with herpesvirus-like virions. A virus with identical ultrastructural characteristics was recovered from cells inoculated with lesion tissues. Partial DNA polymerase gene sequencing placed the virus within the Family Alloherpesviridae, with high similarity to a lake sturgeon herpesvirus (LSHV) from Wisconsin, USA. Genomic comparisons revealed ~84% Average Nucleotide Identity between the two isolates, leading to the proposed classification of LSHV-1 (Wisconsin) and LSHV-2 (Michigan) for the two viruses. When naïve juvenile LST were immersion-exposed to LSHV-2, severe disease and ~33% mortality occurred, with virus re-isolated from representative skin lesions, fulfilling Rivers’ postulates. Results collectively show LSHV-2 is associated with epithelial changes in wild adult LST, disease and mortality in juvenile LST, and is a potential threat to GL-LST conservation.

SUBMITTER: Johnston AE

PROVIDER: S-EPMC9740441 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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First Isolation of a Herpesvirus (Family <i>Alloherpesviridae</i>) from Great Lakes Lake Sturgeon (<i>Acipenser fulvescens</i>).

Johnston Amber E AE Shavalier Megan A MA Scribner Kim T KT Soto Esteban E Griffin Matt J MJ Waldbieser Geoffrey C GC Richardson Bradley M BM Winters Andrew D AD Yun Susan S Baker Edward A EA Larson Douglas L DL Kiupel Matti M Loch Thomas P TP

Animals : an open access journal from MDPI 20221122 23

The lake sturgeon (Acipenser fulvescens; LST) is the only native sturgeon species in the Great Lakes (GL), but due to multiple factors, their current populations are estimated to be <1% of historical abundances. Little is known about infectious diseases affecting GL-LST in hatchery and wild settings. Therefore, a two-year disease surveillance study was undertaken, resulting in the detection and first in vitro isolation of a herpesvirus from grossly apparent cutaneous lesions in wild adult LST in ...[more]

PMID: 36496751

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Project description:Environmental factors play an important role in phenotypic development of fishes, which has implications for hatchery-reared fishes that are released into the wild where natural cues are present. There is interest in examining how early exposure to dietary odourants can affect development of olfaction. The aim of our study was to use behavioural, molecular and electro-physiological techniques to evaluate how introduction of the amino acid L-alanine to the rearing environment might influence the development of olfactory perception of dietary cues, growth and survival in lake sturgeon (Acipenser fulvescens), a species of conservation concern. We hypothesized that exposure to amino acids would influence the onset of feeding during dietary transitions from endogenous to exogenous feeding and predicted that the introduction of L-alanine during early development would promote growth and survival of age-0 lake sturgeon. Additionally, we hypothesized that olfaction in lake sturgeon is a developmentally plastic trait, predicting that the addition of L-alanine prior to exogenous feeding would influence mRNA transcript abundance of genes associated with detection of dietary cues. Our approach was to add L-alanine daily from 17 to 20 days post-fertilization (DPF) before the onset of exogenous feeding. We sampled individuals at 17, 21, 26, 31, 50, 65 and 80 DPF. Additionally, olfactory sensitivity to L-alanine was tested at ~1 year via electro-olfactogram (EOG). We observed no significant differences in mortality or EOG response between L-alanine and control treatments; however, significant differences were observed in morphometrics, behaviour and mRNA transcript abundance of all genes throughout development. Our results indicated the olfactory system exhibited developmental plasticity in response to L-alanine treatment until 50-65 DPF, suggesting that environmental odourants may influence early development of key olfactory processes. Our data could inform practises at conservation hatcheries that are used as part of enhancement programmes for lake sturgeon.

Dataset Information

First Isolation of a Herpesvirus (Family Alloherpesviridae) from Great Lakes Lake Sturgeon (Acipenser fulvescens).

Publications

First Isolation of a Herpesvirus (Family <i>Alloherpesviridae</i>) from Great Lakes Lake Sturgeon (<i>Acipenser fulvescens</i>).

Similar Datasets

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