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Response Surface Modeling and Optimization of the Extraction of Phenolic Antioxidants from Olive Mill Pomace.

ABSTRACT: Bioactive compounds from olive mill pomace (OMP) were extracted through a two-step solid-liquid extraction procedure considering four factors at five levels of a central composite rotatable response surface design. The influence of the process variables time of the primary extraction (2.0-4.0 h), solvent-to-sample ratio during the primary extraction (5.0-10.0 mL/g), time of the secondary extraction (1.0-2.0 h), and the solvent-to-sample ratio during the secondary extraction (3.0-5.0 mL/g) were examined. The content of bioactive compounds was determined spectrophotometrically, and the individual phenolic compounds were evaluated by reserved-phase high-performance liquid chromatography (RP-HPLC). The Derringer's function was used to optimize the extraction process, and the best conditions were found to be 3.2 h for the primary extraction, 10.0 mL/g for the solvent-to-sample ratio and 1.3 h for the secondary extraction associated with a solvent-to-sample ratio of 3.0 mL/g, obtaining a total phenolic content of 50.0 (expressed as mg gallic acid equivalents (GAE)/g dry weight (dw). The response surface methodology proved to be a great alternative for reducing the number of tests, allowing the optimization of the extraction of phenolic antioxidants from OMP with a reduced number of experiments, promoting reductions in cost and analysis time.

SUBMITTER: Paulo F

PROVIDER: S-EPMC9741320 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Response Surface Modeling and Optimization of the Extraction of Phenolic Antioxidants from Olive Mill Pomace.

Paulo Filipa F Tavares Loleny L Santos Lúcia L

Molecules (Basel, Switzerland) 20221206 23

Bioactive compounds from olive mill pomace (OMP) were extracted through a two-step solid-liquid extraction procedure considering four factors at five levels of a central composite rotatable response surface design. The influence of the process variables time of the primary extraction (2.0-4.0 h), solvent-to-sample ratio during the primary extraction (5.0-10.0 mL/g), time of the secondary extraction (1.0-2.0 h), and the solvent-to-sample ratio during the secondary extraction (3.0-5.0 mL/g) were e ...[more]

PMID: 36500712

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Project description:BackgroundThe application of Pulsed electric fields as a mild and easily scalable electrotechnology represents an effective approach to selectively intensify the extractability of bioactive compounds from grape pomace, one of the most abundant residues generated during the winemaking process.ObjectiveThis study addressed the optimization of the pulsed electric fields (PEF)-assisted extraction to enhance the extraction yields of bioactive compounds from red grape pomace using response surface methodology (RSM).MethodsThe cell disintegration index (Z p ) was identified as response variable to determine the optimal PEF processing conditions in terms of field strength (E = 0.5-5 kV/cm) and energy input (WT = 1-20 kJ/kg). For the solid-liquid extraction (SLE) process the effects of temperature (20-50°C), time (30-300min), and solvent concentration (0-50% ethanol in water) on total phenolic content (TPC), flavonoid content (FC), total anthocyanin content (TAC), tannin content (TC), and antioxidant activity (FRAP) of the extracts from untreated and PEF-treated plant tissues were assessed. The phenolic composition of the obtained extracts was determined via HPLC-PDA.ResultsResults demonstrated that the application of PEF at the optimal processing conditions (E = 4.6 kV/cm, WT = 20 kJ/kg) significantly enhanced the permeabilization degree of cell membrane of grape pomace tissues, thus intensifying the subsequent extractability of TPC (15%), FC (60%), TAC (23%), TC (42%), and FRAP values (31%) concerning the control extraction. HPLC-PDA analyses showed that, regardless of the application of PEF, the most abundant phenolic compounds were epicatechin, p-coumaric acid, and peonidin 3-O-glucoside, and no degradation of the specific compounds occurred upon PEF application.ConclusionThe optimization of the PEF-assisted extraction process allowed to significantly enhance the extraction yields of high-value-added compounds from red grape pomace, supporting further investigations of this process at a larger scale.

Dataset Information

Response Surface Modeling and Optimization of the Extraction of Phenolic Antioxidants from Olive Mill Pomace.

Publications

Response Surface Modeling and Optimization of the Extraction of Phenolic Antioxidants from Olive Mill Pomace.

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