FbpABC gene cluster in Neisseria meningitidis is transcribed as an operon.
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ABSTRACT:
SUBMITTER: Khun HH
PROVIDER: S-EPMC97834 | biostudies-literature | 2000 Dec
REPOSITORIES: biostudies-literature
Publications
fbpABC gene cluster in Neisseria meningitidis is transcribed as an operon.
Khun H H HH Deved V V Wong H H Lee B C BC
Infection and immunity 20001201 12
The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with an fbpC-sequence-specific oligonucleotide indicates that fbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-f ...[more]
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