Dataset Information

Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability.

ABSTRACT:

Aims

Diets containing high-fat and high sugar (HFHS) lead to overweight/obesity. Overweight/obesity increases the risk of infertility, and of the pregnant mother and her child for developing metabolic conditions. Overweight/obesity has been recreated in mice, but most studies focus on the effects of chronic, long-term HFHS diet exposure. Here, we exposed mice to HFHS from 3 weeks prior to pregnancy with the aim of determining impacts on fertility, and gestational and neonatal outcomes.

Methods

Time-domain NMR scanning was used to assess adiposity, glucose, and insulin tolerance tests were employed to examine metabolic physiology, and morphological and proteomic analyses conducted to assess structure and nutrient levels of maternal organs and placenta.

Results

Fertility measures of HFHS dams were largely the same as controls. HFHS dams had increased adiposity pre-pregnancy, however, exhibited exacerbated lipolysis/hyper-mobilization of adipose stores in late pregnancy. While there were no differences in glucose or insulin tolerance, HFHS dams were hyperglycemic and hyperinsulinemic in pregnancy. HFHS dams had fatty livers and altered pancreatic islet morphology. Although fetuses were hyperglycemic and hyperinsulinemic, there was no change in fetal growth in HFHS dams. There were also reductions in placenta formation. Moreover, there was increased offspring loss during lactation, which was related to aberrant mammary gland development and milk protein composition in HFHS dams.

Conclusions

These findings are relevant given current dietary habits and the development of maternal and offspring alterations in the absence of an increase in maternal weight and adiposity during pregnancy, which are the current clinical markers to determine risk across gestation.

SUBMITTER: Lean SC

PROVIDER: S-EPMC9787084 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability.

Lean Samantha C SC Candia Alejandro A AA Gulacsi Edina E Lee Giselle C L GCL Sferruzzi-Perri Amanda N AN

Acta physiologica (Oxford, England) 20220803 2

<h4>Aims</h4>Diets containing high-fat and high sugar (HFHS) lead to overweight/obesity. Overweight/obesity increases the risk of infertility, and of the pregnant mother and her child for developing metabolic conditions. Overweight/obesity has been recreated in mice, but most studies focus on the effects of chronic, long-term HFHS diet exposure. Here, we exposed mice to HFHS from 3 weeks prior to pregnancy with the aim of determining impacts on fertility, and gestational and neonatal outcomes.<h ...[more]

PMID: 35880402

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Dataset Information

Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability.

Aims

Methods

Results

Conclusions

Publications

Obesogenic diet in mice compromises maternal metabolic physiology and lactation ability leading to reductions in neonatal viability.

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