Dataset Information

Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides.

ABSTRACT: The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation. Immunolocalisation identified remodelling of pectin towards de-esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM-induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper. This suggests that LbPME1 plays a role in ECM formation potentially through HG de-esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.

SUBMITTER: Chowdhury J

PROVIDER: S-EPMC9796311 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides.

Chowdhury Jamil J Kemppainen Minna M Delhomme Nicolas N Shutava Iryna I Zhou Jingjing J Takahashi Junko J Pardo Alejandro G AG Lundberg-Felten Judith J

The New phytologist 20220727 2

The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin-mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides. We combine transcriptomics of cell-wall-related enzymes in both partn ...[more]

PMID: 35794841

Similar Datasets

Project description:Fungi of the genus Trichoderma are economically important due to their plant growth- and performance-promoting effects, such as improved nutrient supply, mycoparasitism of plant-pathogens and priming of plant defense. Due to their mycotrophic lifestyle, however, they might also be antagonistic to other plant-beneficial fungi, such as mycorrhiza-forming species. Trichoderma spp. release a high diversity of volatile organic compounds (VOCs), which likely play a decisive role in the inter-species communication. It has been shown that Trichoderma VOCs can inhibit growth of some plant pathogens, but their inhibition potentials during early interactions with mutualistic fungi remain unknown. Laccaria bicolor is a common ectomycorrhizal fungus which in symbiotic relationship is well known to facilitate plant performance. Here, we investigated the VOC profiles of three strains of Trichoderma species, Trichoderma harzianum, Trichoderma Hamatum, and Trichoderma velutinum, as well as L. bicolor by stir bar sorptive extraction and gas chromatography - mass spectrometry (SBSE-GC-MS). We further examined the fungal performance and the VOC emission profiles during confrontation of the Trichoderma species with L. bicolor in different co-cultivation scenarios. The VOC profiles of the three Trichoderma species were highly species-dependent. T. harzianum was the strongest VOC emitter with the most diverse compound pattern, followed by T. hamatum and T. velutinum. Co-cultivation of Trichoderma spp. and L. bicolor altered the VOC emission patterns dramatically in some scenarios. The co-cultivations also revealed contact degree-dependent inhibition of one of the fungal partners. Trichoderma growth was at least partially inhibited when sharing the same headspace with L. bicolor. In direct contact between both mycelia, however, L. bicolor growth was impaired, indicating that Trichoderma and L. bicolor apply different effectors when defending their territory. Multivariate analysis demonstrated that all examined individual fungal species in axenic cultures, as well as their co-cultivations were characterized by a distinct VOC emission pattern. The results underline the importance of VOCs in fungal interactions and reveal unexpected adjustability of the VOC emissions according to the specific biotic environments.

Project description:This study was conducted to assess the survival rates, growth, and chlorophyll fluorescence (Fv/Fm) of four hybrid aspen (14, 191, 27, 291) and two European aspen (R3 and R4) clones cultivated in creosote- and diesel oil-contaminated soil treatments under three different plant densities: one plant per pot (low density), two plants per pot (medium density), and six plants per pot (high density) over a period of two years and three months. Evaluating the survival, growth, and Fv/Fm values of different plants is a prerequisite for phytoremediation to remediate polluted soils for ecological restoration and soil health. The results revealed that contaminated soils affected all plants' survival rates and growth. However, plants grown in the creosote-contaminated soil displayed a 99% survival rate, whereas plants cultivated in the diesel-contaminated soil showed a 22-59% survival rate. Low plant density resulted in a higher survival rate and growth than in the other two density treatments. In contrast, the medium- and high-density treatments did not affect the plant survival rate and growth to a greater extent, particularly in contaminated soil treatments. The effects of clonal variation on the survival rate, growth, and Fv/Fm values were evident in all treatments. The results suggested that hybrid aspen clones 14 and 291, and European aspen clone R3 were suitable candidates for the phytoremediation experiment, as they demonstrated reasonable survival rates, growth, and Fv/Fm values across all treatments. A superior survival rate for clone 291, height and diameter growth, and stem dry biomass production for clone 14 were observed in all soil treatments. Overall, a reasonable survival rate (~75%) and Fv/Fm value (>0.75) for all plants in all treatments, indicating European aspen and hybrid aspen have considerable potential for phytoremediation experiments. As the experiment was set up for a limited period, this study deserves further research to verify the growth potential of different hybrid aspen and European aspen clones in different soil and density treatment for the effective phytoremediation process to remediate the contaminated soil.

Dataset Information

Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides.

Publications

Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets