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Analysis of mitochondrial double-stranded RNAs in human cells.

ABSTRACT: Human mitochondrial genome is transcribed bidirectionally, generating long complementary RNAs that can form double-stranded RNAs (mt-dsRNAs). When released to the cytosol, these mt-dsRNAs can activate antiviral signaling. Here, we present a detailed protocol for the analysis of mt-dsRNA expression. The protocol provides three approaches that can complement one another in examining mt-dsRNAs. While the described protocol is optimized for human cells, this approach can be adapted for use in other animal cell lines and tissue samples. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).¹.

SUBMITTER: Kim S

PROVIDER: S-EPMC9850877 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Analysis of mitochondrial double-stranded RNAs in human cells.

Kim Sujin S Yoon Jimin J Lee Keonyong K Kim Yoosik Y

STAR protocols 20230119 1

Human mitochondrial genome is transcribed bidirectionally, generating long complementary RNAs that can form double-stranded RNAs (mt-dsRNAs). When released to the cytosol, these mt-dsRNAs can activate antiviral signaling. Here, we present a detailed protocol for the analysis of mt-dsRNA expression. The protocol provides three approaches that can complement one another in examining mt-dsRNAs. While the described protocol is optimized for human cells, this approach can be adapted for use in other ...[more]

PMID: 36853732

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