Ontology highlight
ABSTRACT:
SUBMITTER: Tachioka M
PROVIDER: S-EPMC9898803 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Tachioka Mikako M Tsudome Mikiko M Deguchi Shigeru S
STAR protocols 20230124 1
Assaying enzymatic degradation of water-insoluble substrates like cellulose is challenging because only the substrate surface is accessible to the enzymes resulting in low reaction rates. Here, we describe a protocol for surface pitting observation technology (SPOT), an ultra-sensitive quantitative assay for analyzing enzymatic hydrolysis of cellulose. We describe the use of a porous substrate to accelerate the hydrolysis rate of cellulose. We also detail the steps for combining inkjet patternin ...[more]