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Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics.

ABSTRACT: Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%-116.32%), precision (covariance <20%), sensitivity (LOD <0.1 μg/mL), turnaround time (<5 minutes), and linearity (R² = 0.9827-0.9992) within 0.1-100 μg/mL.

SUBMITTER: Su H

PROVIDER: S-EPMC9930999 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics.

Su Hung H Lin Hsin-Hua HH Su Lin-Jhen LJ Lin Chia-Chen CC Jiang Zong-Han ZH Chen Shiang-Jin SJ Shiea Jentaie J Lee Chi-Wei CW

Journal of food and drug analysis 20220315 1

Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care pl ...[more]

PMID: 35647718

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