Dataset Information

Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.

ABSTRACT: In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. Rousettus aegyptiacus (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017-2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus RNA was identified from one fecal sample out of 860 samples. Genome segments were recovered using segment-specific amplification combined with standard Sanger sequencing and Illumina unbiased sequencing. The identified influenza A virus was closely related to the H9N2 bat-influenza virus, confirming the circulation of this subtype among Egyptian fruit bat populations in Southern Africa. This bat H9N2 subtype contained amino acid residues associated with transmission and virulence in either mammalian or avian hosts, though it will likely require additional adaptations before spillover.

SUBMITTER: Rademan R

PROVIDER: S-EPMC9958621 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.

Rademan Rochelle R Geldenhuys Marike M Markotter Wanda W

Viruses 20230210 2

In recent years, bats have been shown to host various novel bat-specific influenza viruses, including H17N10 and H18N11 in the Americas and the H9N2 subtype from Africa. <i>Rousettus aegyptiacus</i> (Egyptian Rousette bat) is recognized as a host species for diverse viral agents. This study focused on the molecular surveillance of a maternal colony in Limpopo, South Africa, between 2017-2018. A pan-influenza hemi-nested RT-PCR assay targeting the PB1 gene was established, and influenza A virus R ...[more]

PMID: 36851712

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Project description:The study was conducted to phenotypically characterize Nguni goats from four agro-ecological zones of Limpopo province, South Africa. A total of 426 goats were sampled from four agro-ecological zones. The quantitative traits that were studied for phenotypic characterization using Analysis of Variance (ANOVA) were heart girth (HG), body length (BL), withers height (WH), sternum height (SH), rump height (RH), rump length (RL), rump width (RW), cannon circumference (CC), testicular length (TL) and scrotal circumference (SC) while qualitative traits were coat colour pattern and type, back profile, ear orientation, presence of horn, horn shape and orientation. The study further looked at the structural indices of the goats. The findings indicated that the agro-ecological zones significantly affected (P<0.05) the quantitative traits of Nguni goats. Female goats from arid zone had higher (P<0.05) BW, HG, BL, WH, RH, and CC with mean values of 35.76±0.92, 73.89±0.63, 66.26±0.62, 63.40±0.56, 64.71±0.44 and 8.07±0.06, respectively as compared to the other zones. Male goats from arid zone had higher (P<0.05) BW, HG, BL, RW, TL and SC with mean values of 37.20±2.29, 78.75±1.44, 70.95±1.57, 16.83±0.38, 15.50±0.50 and 31.00±0.00, respectively as compared to other zones. In terms of qualitative traits, the goats were characterized by higher proportion of patchy coat colour pattern (62.7%), brown dominant colour type (42.6%), straight back profile (46.6%), and semi-pendulous ear orientation (65.4%). The goats had horns (100%), curved horn shape (71.6%) and backward horn orientation (89.4%). Though the agro-ecological zone had non-significant influence (P>0.05) on the qualitative traits. Findings of structural indices on dactyl-thoracic, transversal pelvic, longitudinal pelvic and proportionality indicated that the Nguni goats can be matched as light medium meat type. In conclusion, the variation in morphometric traits of Nguni goats and knowing their structural and functional indices can assist in their conservation and genetic improvement. There was no difference in the qualitative traits of the goats in four agro-ecological zones. Studies needed to be conducted on genetic characterization using genetic markers to integrate the information from morphological traits.

Dataset Information

Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.

Publications

Detection and Characterization of an H9N2 Influenza A Virus in the Egyptian Rousette Bat in Limpopo, South Africa.

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