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Structure of TRPV1 in complex with DkTx and RTX, determined in lipid nanodisc

ABSTRACT:

SUBMITTER: Yuan Gao

PROVIDER: EMPIAR-10059 | biostudies-other |

REPOSITORIES: biostudies-other

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TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action.

Gao Yuan Y Cao Erhu E Julius David D Cheng Yifan Y

Nature 20160518 7607

When integral membrane proteins are visualized in detergents or other artificial systems, an important layer of information is lost regarding lipid interactions and their effects on protein structure. This is especially relevant to proteins for which lipids have both structural and regulatory roles. Here we demonstrate the power of combining electron cryo-microscopy with lipid nanodisc technology to ascertain the structure of the rat TRPV1 ion channel in a native bilayer environment. Using this ...[more]

PMID: 27281200

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