Cryo-electron tomography of immature HIV-1 dMACANC VLPs
Ontology highlight
ABSTRACT:
SUBMITTER: Florian Schur
PROVIDER: EMPIAR-10164 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation.
Schur Florian K M FK Obr Martin M Hagen Wim J H WJ Wan William W Jakobi Arjen J AJ Kirkpatrick Joanna M JM Sachse Carsten C Kräusslich Hans-Georg HG Briggs John A G JA
Science (New York, N.Y.) 20160714 6298
Immature HIV-1 assembles at and buds from the plasma membrane before proteolytic cleavage of the viral Gag polyprotein induces structural maturation. Maturation can be blocked by maturation inhibitors (MIs), thereby abolishing infectivity. The CA (capsid) and SP1 (spacer peptide 1) region of Gag is the key regulator of assembly and maturation and is the target of MIs. We applied optimized cryo-electron tomography and subtomogram averaging to resolve this region within assembled immature HIV-1 pa ...[more]
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