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Rabbit muscle aldolase single particle cryoEM

ABSTRACT:

SUBMITTER: Alex Noble

PROVIDER: EMPIAR-10215 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs.

Bepler Tristan T Morin Andrew A Rapp Micah M Brasch Julia J Shapiro Lawrence L Noble Alex J AJ Berger Bonnie B

Nature methods 20191007 11

Cryo-electron microscopy is a popular method for the determination of protein structures; however, identifying a sufficient number of particles for analysis can take months of manual effort. Current computational approaches find many false positives and require ad hoc postprocessing, especially for unusually shaped particles. To address these shortcomings, we develop Topaz, an efficient and accurate particle-picking pipeline using neural networks trained with a general-purpose positive-unlabeled ...[more]

PMID: 31591578

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