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Single particle cryo electron microscopy of aldolase (rabbit, muscle) using beam-tilt on Talos Arctica

ABSTRACT:

SUBMITTER: Sarah Kearns

PROVIDER: EMPIAR-10519 | biostudies-other |

REPOSITORIES: biostudies-other

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High-resolution cryo-EM using beam-image shift at 200 keV.

Cash Jennifer N JN Kearns Sarah S Li Yilai Y Cianfrocco Michael A MA

IUCrJ 20201029 Pt 6

Recent advances in single-particle cryo-electron microscopy (cryo-EM) data collection utilize beam-image shift to improve throughput. Despite implementation on 300 keV cryo-EM instruments, it remains unknown how well beam-image-shift data collection affects data quality on 200 keV instruments and the extent to which aberrations can be computationally corrected. To test this, a cryo-EM data set for aldolase was collected at 200 keV using beam-image shift and analyzed. This analysis shows that the ...[more]

PMID: 33209328

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