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Sub-3 Å Apoferritin Structure Determined With Full Range of Phase Shifts Using A Single Position Of Volta Phase Plate

ABSTRACT:

SUBMITTER: Wen Jiang

PROVIDER: EMPIAR-10263 | biostudies-other |

REPOSITORIES: biostudies-other

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Sub-3 Å apoferritin structure determined with full range of phase shifts using a single position of volta phase plate.

Li Kunpeng K Sun Chen C Klose Thomas T Irimia-Dominguez Jose J Vago Frank S FS Vidal Ruben R Jiang Wen W

Journal of structural biology 20190327 2

Volta Phase Plate (VPP) has become an invaluable tool for cryo-EM structural determination of small protein complexes by increasing image contrast. Currently, the standard protocol of VPP usage periodically changes the VPP position to a fresh spot during data collection. Such a protocol was to target the phase shifts to a relatively narrow range (around 90°) based on the observations of increased phase shifts and image blur associated with more images taken with a single VPP position. Here, we r ...[more]

PMID: 30928614

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Sub-3 A apoferritin structure determined with full range of phase shifts using a single position of volta phase plate.

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