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Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein

ABSTRACT:

SUBMITTER: Christopher Tate

PROVIDER: EMPIAR-10309 | biostudies-other |

REPOSITORIES: biostudies-other

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Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein.

García-Nafría Javier J Lee Yang Y Bai Xiaochen X Carpenter Byron B Tate Christopher G CG

eLife 20180504

The adenosine A2A receptor (A2AR) is a prototypical G protein-coupled receptor (GPCR) that couples to the heterotrimeric G protein GS. Here, we determine the structure by electron cryo-microscopy (cryo-EM) of A2AR at pH 7.5 bound to the small molecule agonist NECA and coupled to an engineered heterotrimeric G protein, which contains mini-GS, the βγ subunits and nanobody Nb35. Most regions of the complex have a resolution of ~3.8 Å or better. ...[more]

PMID: 29726815

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