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FIB-SEM of parapodia from Platynereis dumerilii


ABSTRACT:

SUBMITTER: Yannick Schwab 

PROVIDER: EMPIAR-10310 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

AMST: Alignment to Median Smoothed Template for Focused Ion Beam Scanning Electron Microscopy Image Stacks.

Hennies Julian J   Lleti José Miguel Serra JMS   Schieber Nicole L NL   Templin Rachel M RM   Steyer Anna M AM   Schwab Yannick Y  

Scientific reports 20200206 1


Alignment of stacks of serial images generated by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) is generally performed using translations only, either through slice-by-slice alignments with SIFT or alignment by template matching. However, limitations of these methods are two-fold: the introduction of a bias along the dataset in the z-direction which seriously alters the morphology of observed organelles and a missing compensation for pixel size variations inherent to the image acquisit  ...[more]

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