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Cryo-electron tomography of E. coli minicells

ABSTRACT:

SUBMITTER: Alister Burt

PROVIDER: EMPIAR-10364 | biostudies-other |

REPOSITORIES: biostudies-other

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Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain.

Burt Alister A Cassidy C Keith CK Ames Peter P Bacia-Verloop Maria M Baulard Megghane M Huard Karine K Luthey-Schulten Zaida Z Desfosses Ambroise A Stansfeld Phillip J PJ Margolin William W Parkinson John S JS Gutsche Irina I

Nature communications 20200206 1

Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a t ...[more]

PMID: 32029744

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