Dataset Information
Cryo-electron tomography of Cryo-FIB milled dividing E. coli.
Ontology highlight
ABSTRACT:
SUBMITTER: Paula Navarro
PROVIDER: EMPIAR-11090 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cell wall synthesis and remodelling dynamics determine division site architecture and cell shape in Escherichia coli.
Nature microbiology 20220912 10
The bacterial division apparatus catalyses the synthesis and remodelling of septal peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles. Understanding of this essential process has been limited by the lack of native three-dimensional views of developing septa. Here, we apply state-of-the-art cryogenic electron tomography (cryo-ET) and fluorescence microscopy to visualize the division site architecture and sPG biogenesis dynamics of the Gram-negative bacterium E ...[more]